Borussia Dortmund's Neven Subotic and Roma's Adem Ljajic no longer play a part in Dick Advocaat's plans for Serbia, the Dutchman has told a news conference.
Advocaat, 67, took over Serbia following former coach Sinsa Mihajlovic's failure to qualify for the 2014 World Cup, winning only two of their first eight qualifiers.
Following a short stint under Ljubinko Drulovic, Advocaat -- who has already coached Netherlands, the United Arab Emirates, South Korea, Belgium and Russia -- took charge of the White Eagles in July 2014.
Speaking to the press ahead of Serbia's upcoming 2016 European Championship qualifiers against Armenia and Albania, Advocaat announced that he no longer sees a future for centre-back Subotic, or midfielder Ljajic.
Subotic, 25, who played for the U.S. at youth level but then opted to represent Serbia, already took a break from international football in 2013, when he said that his top priority until the World Cup would be his club.
A few weeks later, the centre-back suffered an anterior cruciate ligament tear, and has only returned to Bundesliga football this season.
"My exchange of mails and text messages with Subotic has left no doubt that he doesn't like playing for the national team," Advocaat told a news conference on Wednesday.
"That being the case, I think it's best to keep players with such an attitude away from the squad to prevent them from disrupting the good atmosphere we have."
Roma midfielder Ljajic, 23, has also been axed from the Serbia team once again, having only returned to the team under Drulovic.
In May 2012, under the tenure of Mihajlovic, he was dropped from the national team when he refused to sing the national anthem ahead of a friendly against Spain, despite signing a code of conduct which includes, among other things, doing so prior to kick-off.
"There are some things over which I cannot move, and one of them was the not singing of the national anthem," Mihajlovic said at the time.
"Ljajic is a good player but he has a different mentality and I am looking for players with the right mentality," Advocaat explained.
"So far I've seen that almost everyone is happy to be in the squad and I have no need for players who don't have a good feeling about the national team."