Wales midfielder Aaron Ramsey has been ruled out of his country's first Euro 2020 qualifier at home to Slovakia on Sunday.
Arsenal's Ramsey, 28, has withdrawn from the squad and gone back to London after failing to recover from a thigh injury.
The Wales Twitter account said: "Ryan Giggs has confirmed that @aaronramsey has withdrawn from the @Cymru squad through injury and has returned to @Arsenal for treatment."
🏴 SQUAD UPDATE 🏴— Wales 🏴 (@Cymru) March 23, 2019
Ryan Giggs has confirmed that @aaronramsey has withdrawn from the @Cymru squad through injury and has returned to @Arsenal for treatment.
Brysia wella! 💪#WALSVK pic.twitter.com/Sxyq1KzBaC
Manager Giggs told a news conference: "We have plan B and we have worked on it during the week.
"He was in good form, but it gives a chance for someone else."
Ramsey had missed training ahead of the Slovakia game, with the injury having ruled him out of Wednesday's 1-0 friendly win over Trinidad and Tobago.
Wales star Gareth Bale backed the team to overcome Ramsey's absence, with the Real Madrid winger saying he was "ready to go."
"Obviously it's a big blow for us," Bale said when asked about the news that Ramsey would not be playing. "He's a big player for us and we'll obviously miss him.
"But it gives someone else a chance to step in and do a good job, and hopefully help the team to a win.
"I am fully fit and ready to go. I am enjoying being here and I am excited for the new campaign. I want to keep doing as much as I can to help the team, and so does every player."