The move was made Wednesday, a day after Calhoun aggravated his quad as a baserunner when trying to beat a throw to first base in the sixth inning of a win over Seattle.
Delino DeShields, the center fielder sent to the minors two weeks ago, was recalled from Triple-A Nashville.
Calhoun hit .435 (10-for-23) with two homers and seven RBIs since making his season debut a week ago following his call-up from the minors. He reached safely in all six games.
Manager Chris Woodward said Calhoun initially thought he had a cramp, but the pain didn't go away after getting to the dugout. The outfielder was reexamined Wednesday.