Showing off his opera skills by singing "Ave Maria," Tucker was the winner of "Most Valuable Performer," an NFL talent show televised on CBS on Thursday night.
"This is amazing," Tucker said. "What an incredible opportunity. Such a blast."
Tucker, who earned a music degree at the University of Texas, will give his $50,000 prize money to the Baltimore School for the Arts.
"Hopefully, we're going to keep encouraging Baltimore's youth to stay involved in music, dance and all of the fine arts," he said.
In what was his fourth live performance, Tucker wowed the judges in the first such talent show involving NFL players.
"That's what we call pitch perfect," said Katharine McPhee, a singer and actress.
Tucker, who has sung previously at Christmas concerts, was the star of the show.
"I expected to be impressed tonight," said wide receiver Brandon Marshall, who was also a judge. "But I was not expecting to be moved. This was emotional."